Evaluation of the crude chitinases toxicity on the reproductive system of Callosobruchus maculatus (Coleoptera: Bruchidae)

Laribi-Habchi  Hassiba; Amrouche Zoheir; Boudjamaa Nouara; Mohamed Mahmoud Fadhila; Medjdoub  Ouafaa; Akroum-Amrouche  Dahbia; Akroum  Hamza

doi:10.59400/jts.v2i2.1393

Laribi-Habchi Hassiba Laboratory of Functional Analysis of Chemical Process, University Saad Dahlab of Blida, Blida 09000, Algeria; Loboratory of Energy Applications of Hydrogen, University Saad Dahlab of Blida, Blida 09000, Algeria
Amrouche Zoheir Laboratory of Functional Analysis of Chemical Process, University Saad Dahlab of Blida, Blida 09000, Algeria; Biological Department, Faculty of Nature and Life Science, University of Khemis Miliana, Ain-Defla 44003, Algeria
Boudjamaa Nouara Biological Department, Faculty of Natural and Life Sciences (NLS), University Saad Dahlab Blida, Blida 09000, Algeria
Mohamed Mahmoud Fadhila Laboratory for the Protection and Valorization of Agro Biological Resources, Faculty of Natural and Life Sciences (NLS), University Saad Dahlab Blida, Blida 09000, Algeria
Medjdoub Ouafaa Loboratory of Energy Applications of Hydrogen, University Saad Dahlab of Blida, Blida 09000, Algeria
Akroum-Amrouche Dahbia Bioinformatics and Applied Microbiology and Bio Molecules Laboratory, Faculty of Sciences, M’Hamed Bougara University, Boumerdes 35000, Algeria
Akroum Hamza Applied Automatic Laboratory, Faculty of Hydrocarbons and Chemistry, M’Hamed Bougara University, Boumerdes 35000, Algeria

Article ID: 1393

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DOI: https://doi.org/10.59400/jts.v2i2.1393

Keywords: chitinase; Scorpaena scrofa; bioinsecticide; stored foods; Callosobruchus maculatus

Abstract

Insecticides represent the most used control method against the insect pests of stored food products. But there are strains of insects that are resistant to these insecticides; this is the case of the beetle of the chickpea, Callosobruchus maculatus. The present study has for objective the evaluation of the biological activity of chitinolytic enzymes extracted from the fish offals Scorpaena scrofa (scorpion fish) on this beetle at different doses (3%, 6%, 9%, 12%, 15%, and 21%) prepared with the buffer solution. The results obtained from three treatments (T₁, T₂, and T₃) realized according to the dose of the crude enzyme and the time of exposure were compared with those of the controls and have shown a very significant efficiency of our crude enzyme on the reduction of the fertility rate of 100% by treating the couple (T₁) at the same time as to treat the male (T₃) and the female (T₂) separately with the strongest dose (21%) for 48 h of exposure. It would be thus desirable to demonstrate the activity of these enzymes in the real conditions of storage.

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